1235572-56-1

Basic information

• Product Name: C₈₇H₁₀₂N₂₀O₁₁
• Synonyms: C₈₇H₁₀₂N₂₀O₁₁
• CAS NO: 1235572-56-1
• Molecular Weight: 1603.89
• Mol File: 1235572-56-1.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: C₈₇H₁₀₂N₂₀O₁₁(CAS DataBase Reference)
• NIST Chemistry Reference: C₈₇H₁₀₂N₂₀O₁₁(1235572-56-1)
• EPA Substance Registry System: C₈₇H₁₀₂N₂₀O₁₁(1235572-56-1)

Safety Data

• Hazardous Substances Data: 1235572-56-1(Hazardous Substances Data)

Upstream product

• 909723-87-1 [2-(3-{3,5-bis-[(bis-pyridin-2-ylmethyl-amino)-methyl]-4-hydroxy-phenyl}-2-tert-butoxycarbonylamino-propionylamino)-acetylamino]-acetic acid 
• 1004751-65-8 C₄₃H₅₃N₁₁O₅ 

Downstream Products

• 1235572-57-2 C₈₂H₉₄N₂₀O₉ 

Relevant articles and documents

Selective covalent labeling of tag-fused GPCR proteins on live cell surface with a synthetic probe for their functional analysis

Selective protein labeling with a small molecular probe is a versatile method for elucidating protein functions in living cells. In this paper, we report a covalent labeling method of tag-fused G-protein coupled receptor (GPCR) proteins expressing on cell surfaces utilizing small functional molecules. This method employs the selective and rapid reaction of a peptide tag and a molecular probe, which comprises the cysteine-containing short CA6D4×2 tag (CAAAAAADDDDGDDDD) and a tetranuclear Zn(II)-DpaTyr probe containing a reactive α-chloroacetyl moiety. The covalent labeling of tag-fused GPCRs such as bradykinin receptor (B2R) and acetylcholine receptor (m1AchR) selectively proceeded under physiological conditions during short incubation (10-30 min) with Zn(II)-DpaTyr probes bearing various functional groups. Labeling with fluorophore-appended Zn(II)-DpaTyr probes enabled visualization of the GPCRs on the surface of HEK293 cells by fluorescence. Labeling with the biotin-appended probe allowed introduction of a biotin unit into the GPCRs. This biotin label was utilized for fluorescence bioimaging studies and postlabeling blotting analysis of the labeled GPCRs by use of the specific biotin-streptavidin interaction. The utility of this labeling method was demonstrated in several function analyses of GPCRs, such as fluorescence visualization of the stimuli-responsive internalization of GPCRs and pH change in endosomes containing the internalized GPCRs.