1236005-70-1Relevant academic research and scientific papers
Ligand Exchange Chromatography of Amino Alcohols. Use of Schiff Bases in Enantiomer Resolution
Gelber, Loren R.,Karger, Barry L.,Neumeyer, John L.,Feibush, Binyamin
, p. 7729 - 7734 (2007/10/02)
A new method for enantiomeric resolution of primary amino alcohols by HPLC is described involving derivatization to the salicylaldehyde Schiff base followed by ligand exchange chromatography (LEC) with chiral L-proline-bonded stationary phases.All of the α-amino alcohols with a hydroxyl substituent on the asymmetric carbon examined were resolved, including catecholamines and other β-hydroxyphenethylamines.The copper(II) ion employed in LEC serves to stabilize the Schiff base, whereas the derivative permits complexation in a manner favorable for resolution and enhanced detection.A possible structure for the mixed complex responsible for separation is suggested.From this structure, a correlation between elution order and configuration is proposed.
