1236375-50-0Relevant academic research and scientific papers
Dendritic chain reaction: Responsive release of hydrogen peroxide upon generation and enzymatic oxidation of methanol
Avital-Shmilovici, Michal,Shabat, Doron
experimental part, p. 3643 - 3647 (2010/07/17)
Signal amplification dramatically increases the sensitivity of diagnostic methods. Recently, we introduced a new technique for signal amplification that uses a distinctive dendritic chain reaction (DCR) to generate exponential evolution of a diagnostic signal. In this report, we demonstrate how the modular design of our DCR probe can be used to improve the detection sensitivity. We synthesized a new probe based on a methyl carbonate linkage, which has superior stability in aqueous media. Triggered release of methanol, which was oxidized by alcohol oxidase present in the solution, produced hydrogen peroxide that used as a reagent in the DCR amplification technique. The new probe exhibited higher sensitivity in detection of hydrogen peroxide than our previously reported probe.
