1240125-36-3Relevant academic research and scientific papers
Fluorogenic dendrons with multiple donor chromophores as bright genetically targeted and activated probes
Szent-Gyorgyi, Christopher,Schmidt, Brigitte F.,Fitzpatrick, James A. J.,Bruchez, Marcel P.
, p. 11103 - 11109 (2010)
We have developed a class of dendron-based fluorogenic dyes (termed dyedrons) comprised of multiple cyanine (Cy3) donors coupled to a single malachite green (MG) acceptor that fluoresce only when the MG is noncovalently but specifically bound to a cognate
QUENCHED DENDRIMERIC DYES FOR BRIGHT DETECTION
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Page/Page column 36, (2010/09/17)
The present invention presents designs for high extinction quenched dyedrons that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.
