1241387-99-4Relevant academic research and scientific papers
Convenient RNA synthesis using a phosphoramidite possessing a biotinylated photocleavable group
Tomaya, Kota,Takahashi, Mayumi,Minakawa, Noriaki,Matsuda, Akira
supporting information; experimental part, p. 3836 - 3839 (2010/11/17)
A convenient RNA synthesis using a biotin-streptavidin interaction and a photocleavable protecting group is described. The biotinylated photocleavable group was introduced at the 2′-position of the uridine derivative. Using the phosphoramidite 12, we attempted the synthesis of a 21mer RNA, which is pure enough to show potent RNAi activity compared with a conventionally prepared and HPLC-purified 21mer RNA with the same sequence.
