1245606-72-7Relevant academic research and scientific papers
Simple biosensor with high selectivity and sensitivity: Thiol-specific biomolecular probing and intracellular imaging by AIE fluorogen on a TLC plate through a thiol-ene click mechanism
Liu, Yang,Yu, Yong,Lam, Jacky W. Y.,Hong, Yuning,Faisal, Mahtab,Yuan, Wang Zhang,Tang, Ben Zhong
, p. 8433 - 8438 (2010)
A handy, specific, sensitive bioprobe has been developed. Tetraphenylethene (TPE) was functionalized by a maleimide (MI) group, giving a TPE-MI adduct that was nonemissive in both solution and the solid state. It was readily transformed into a fluorogen showing an aggregation-induced emission (AIE) property by the click addition of thiol to its MI pendant. The click reaction and the AIE effect enabled TPE-MI to function as a thiol-specific bioprobe in the solid state. Thus, the spot of TPE-MI on a TLC plate became emissive when it had been exposed to L-cysteine, an amino acid containing a thiol group, but remained nonemissive when exposed to other amino acids that lack free thiol units. The thiol-activated emission was rapid and strong, readily detected by the naked eye at an analyte concentration as low as approximately 1 ppb, thanks to the lighting up nature of the bioprobing process. Similarly, the emission of TPE-MI was turned on only by the proteins containing free thiol units, such as glutathione. Clear fluorescence images were taken when living cells were stained by using TPE-MI as a visualization agent, affording a facile fluorescent maker for mapping the distribution of thiol species in cellular systems.
Luminogen compounds and the use of the same for biosensing and cellular imaging
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Page/Page column 7; 11; 12; 13, (2016/11/14)
Provided herein are a luminogen compound of formula (I) including a AIE luminophore moiety conjugated with a maleimide moiety and a use of the same for detecting thiol groups in biomolecules. Also provided is a dye molecule, a biosensor or a bioprobe comprising the luminogen compound of formula (I) in use for detecting thiol groups in biomolecules. The detection method of the present subject matter not only has high thio-selectivity and sensitivity, but also is rapid, convenient and handy.
