125328-76-9 Usage
Uses
Used in Dye Formulation:
5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is used as a chemical intermediate for the formulation of dyes, contributing to the development of colorants in various industries.
Used in Enzymology:
5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is used as a substrate in enzymology, specifically for beta-glucuronidase, an enzyme that catalyzes the hydrolysis of glucuronide conjugates. When acted upon by beta-glucuronidase, it breaks down and releases a colored product that can be easily detected.
Used in Scientific Research:
5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is used as a reagent in scientific research, particularly in the study of enzymatic reactions and the detection of beta-glucuronidase activity.
Used in Diagnostic Applications:
5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is used in diagnostic applications, where its enzymatic breakdown and release of a colored product can be easily detected, aiding in the identification and analysis of certain conditions.
Used in Biochemical Research and Development:
5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is used in biochemical research and development, where its properties and reactions are studied to further understand enzymatic processes and develop new applications in the field.
Check Digit Verification of cas no
The CAS Registry Mumber 125328-76-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,5,3,2 and 8 respectively; the second part has 2 digits, 7 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 125328-76:
(8*1)+(7*2)+(6*5)+(5*3)+(4*2)+(3*8)+(2*7)+(1*6)=119
119 % 10 = 9
So 125328-76-9 is a valid CAS Registry Number.
InChI:InChI=1/C10H7BrClNO2/c1-5(14)13-4-8(15)9-7(13)3-2-6(11)10(9)12/h2-4,15H,1H3
125328-76-9Relevant academic research and scientific papers
Synthesis of a 1,2-: Cis -indoxyl galactoside as a chromogenic glycosidase substrate
Nagata, Sakuto,Tomida, Hirotaka,Iwai-Hirose, Haruka,Tanaka, Hide-Nori,Ando, Hiromune,Imamura, Akihiro,Ishida, Hideharu
, p. 28241 - 28247 (2019/09/30)
A synthetically challenging 1,2-cis-indoxyl galactoside, X-α-galactoside, was first prepared in this study using a cyclic ketone indoxyl acceptor and a glycosyl trichloroacetimidate donor to produce an enol glycoside and a 4,6-O-di-tert-butylsilylene-protected galactosyl donor to complete the synthesis. The target compound shows enzyme activity in the presence of α-galactosidase.
Synthesis of guanosine-3′-(5-bromo-4-chloroindol-3-YL)-phosphate (G-3′-BCIP)
Haikal, Abdelfattah
, p. 427 - 431 (2007/10/03)
N-2-Benzoyl-5′-O-dimethoxytrityl-2′-O- tetrahydropyranylguaaosine (5) and 1-acetyl-5-bromo-4-chloroindol-3-yl-3-phosphorodichloridate (7) were synthesized and coupled to give the title compound 9.
