1262843-46-8Relevant articles and documents
Production method of labelled vitamin D2 internal standard compound
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, (2019/10/23)
The invention relates to a production method of a labelled vitamin D2 internal standard compound. The production method of the labelled vitamin D2 internal standard compound comprises the following steps of firstly, subjecting a gamma-amide phosphonium salt compound as shown in a formula (IV) in the specification and an aldehyde as shown in a formula (III) in the specification to a reaction at -40 DEG to 0 DEG C to obtain an intermediate as shown in a formula (V) in the specification; then adding a Boc anhydride and a catalyst, carrying out a reaction, and then adding an isotope labelled methyl Grignard reagent dropwise to obtain an intermediate as shown in a formula (VI) in the specification; and then adding tetrabutylammonium fluoride, and carrying out a reaction to obtain a finished product, namely, the labelled vitamin D2 internal standard compound. The production method of the labelled vitamin D2 internal standard compound has the advantage that the produced labelled vitamin D2 internal standard compound can be used for 25-hydroxyvitamin D2, 1,25-dihydroxyvitamin D2 and tandem mass spectrometry for detecting the content of VD in human serum by means of the 25-hydroxyvitamin D2 and the 1,25-dihydroxyvitamin D2.