1262883-54-4Relevant academic research and scientific papers
Hydrazine visualization fluorescent probe molecule and preparation method thereof
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Paragraph 0031-0037, (2021/09/01)
The invention provides a hydrazine visualization fluorescent probe molecule and a preparation method thereof. The preparation method comprises the steps of mixing compounds of 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 3-R-benzoic acid and fluorescein, conducting reaction completely under the room temperature condition, then adding a dichloromethane solvent to obtain a crude product; and purifying the crude product by using a forward silica gel chromatographic column to obtain a white solid fluorescent probe molecule 3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(3-R benzoate) for detecting hydrazine. When hydrazine reacts with a fluorescent probe molecule ester bond, a recognition part is removed, a fluorescence emission center is separated, fluorescein with strong column green fluorescence emission is generated, and qualitative detection of hydrazine is realized through fluorescence color signal change. The method is simple in preparation process and low in cost, has excellent detection selectivity and sensitivity, can be used for rapidly and qualitatively detecting hydrazine, and has a wide application prospect.
Synthesis of fluorescein aromatic esters in the presence of P 2O5/SiO2 as catalyst and their hydrolysis studies in the presence of lipase
Eshghi, Hossein,Mirzaie, Narges,Asoodeh, Ahmad
experimental part, p. 120 - 126 (2012/01/13)
A series of fluorescein aryl esters were synthesized by the esterification of fluorescein with carboxylic acids in the presence of P2O 5/SiO2 and their hydrolytic properties were investigated. The rate of hydrolysis in the presence or absence of lipase, due to the increase of fluorescein concentration, was measured by monitoring of fluorescence of the solution and correlated with enzyme activity. In addition, the substitute effect on the aromatic ring of fluorescein esters was studied. In contrast to fluorescein diacetate or dibutyrate, fluorescein dibenzoate and fluorescein bis(4-methylbenzoate) were found to be better substrates for the fluorometric assay of lipase with the higher rate of hydrolysis and better Km value, respectively. As little as 9.3 ng mL-1 of lipase can be detected with fluorescein bis(4-methylbenzoate).
