1263319-85-2Relevant academic research and scientific papers
Functional dihydro-1H-imidazole derivatives for MALDI signal enhancement of a lysine-specific chemical modification
Ho, Ming-Yi,Shieh, Yu-Tse,Liao, Chung-Lin,Chen, Yau-Hung,Cheng, Chien-Chung
, p. 987 - 994 (2009)
The enhancement of signal sensitivity and quantification of low-abundance proteins is of great importance in proteomic analysis. The preparation of 2-methoxy-4,5-dihydro-1H-imidazole (MeO-DHIM) derivatives was accomplished by one-step synthesis of O-methyl-isourea. The signal enhancement induced by these attached moieties increases in an order of compound 1 ≈ 4 2 5 ≈ 6 3 in MALDI mass spectrometry. Peptide-compound 3 adduct was approximately 20 times signal enhancement of the unmodified peptide and of 9 times of the peptide-compound 1 adduct. This result demonstrates that the rational designed organic molecules are capable of providing a sensitive tool in the detection of low-abundance proteins in proteomics.
