12650-88-3 Usage
Description
Lysozyme belongs to a group of enzymes that are known as glycoside hydrolases. It is found in animal tissues, organs, serum as well as in tears, nasal secretions, cervical mucus, and the white of avian eggs. Lysozyme breaks the carbonhydrate chains of the bacterial skin, destroying the structural integrity of the cell wall. Thereby, it has antibacterial properties and was one of the first antibiotics studied by Sir Fleming, the discover of penicillin.
Lysozyme is used either alone or in combination with other synergic compounds as an excellent preservative against many food spoiling microorganisms for fruits & vegetables, tofu & bean curd, seafood & meats, wines & sakes. Organic wines use it to reduce sulfites. It is used in non-pasteurized beer. It has been added to baby formula (to aid digestibility) and gastrointestinal treatments for the elderly.? Lysozyme is used in skin care, to cure and prevent acne and bed sores, as well as in optical, dental, and oral conditions.
References
[1] http://ase.tufts.edu/biology/MolecVisual/bio152/rightlyso.html#top
[2] http://www.xtend-life.com/information/ingredients/lysozyme
[3] http://www.bioseutica.com/products/lysozyme
Uses
Different sources of media describe the Uses of 12650-88-3 differently. You can refer to the following data:
1. Catalyzes the hydrolysis of peptidoglycans found in bacterial cell walls. Lysozyme catalyzes is utilized for the hydrolysis of peptidoglycans found in bacterial cell walls. It is used as a precursor in the production of spheroplasts. It is also used as a preservative against many food spoiling microorganisms. It is used in skin care in order to cure and prevent acne and bed sores, dental and oral conditions. It is used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. Further, it is used in non-pasteurized beer.
2. Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.
3. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
General Description
Lysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).
Flammability and Explosibility
Nonflammable
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Check Digit Verification of cas no
The CAS Registry Mumber 12650-88-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,2,6,5 and 0 respectively; the second part has 2 digits, 8 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 12650-88:
(7*1)+(6*2)+(5*6)+(4*5)+(3*0)+(2*8)+(1*8)=93
93 % 10 = 3
So 12650-88-3 is a valid CAS Registry Number.