126910-31-4

Basic information

• Product Name: H-Ala-AFC
• Synonyms: H-Ala-AFC;H-Ala-AFC Trifluoroacetate salt
• CAS NO: 126910-31-4
• Molecular Formula: C13H11F3N2O3
• Molecular Weight: 300.2332496
• Mol File: 126910-31-4.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 435.9±45.0 °C(Predicted)
• Appearance: /
• Density: 1.487±0.06 g/cm3(Predicted)
• PKA: 12.25±0.20(Predicted)
• CAS DataBase Reference: H-Ala-AFC(CAS DataBase Reference)
• NIST Chemistry Reference: H-Ala-AFC(126910-31-4)
• EPA Substance Registry System: H-Ala-AFC(126910-31-4)

Safety Data

• Hazardous Substances Data: 126910-31-4(Hazardous Substances Data)

Usage

General Description

H-Ala-AFC is a synthetic peptide substrate that is commonly used in enzymatic assays to evaluate the activity of proteases, particularly caspases, which play an important role in programmed cell death. This non-fluorescent substrate is cleaved by the target protease, resulting in the release of highly fluorescent AFC (7-amino-4-trifluoromethylcoumarin) that can be easily detected and quantified using a fluorescence plate reader. H-Ala-AFC is widely used in research settings to measure caspase activity in various biological samples, making it a valuable tool for studying apoptosis and other cellular processes that involve protease activation. Its simple design and reliable detection make it a versatile and effective compound for investigating proteolytic activity in the field of biochemistry and molecular biology.