127294-70-6 Usage
Description
Balofloxacin, also known as Q-Roxin, is a novel orally-active fluoroquinolone antibiotic introduced in South Korea for the treatment of urinary tract infections (UTIs). It is synthesized by the reaction of 3-(methylamino)piperidine with the classical 4-quinolone-3-carboxylic acid template. Balofloxacin is a crystalline solid that inhibits the synthesis of bacterial DNA by interfering with the enzyme DNA gyrase.
Uses
Used in Pharmaceutical Industry:
Balofloxacin is used as a fluorinated quinolone antibacterial agent for the treatment of urinary tract infections (UTIs). It is effective against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, Staphylococcus epidermis, Streptococcus pneumonia, and Streptococcus pyrogenes.
Used in Clinical Trials:
Balofloxacin is used as a quinolone antibiotic in clinical trials, demonstrating well-tolerated and comparable efficacy to ofloxacin in patients with UTIs. After oral administration, it is well absorbed and primarily eliminated unchanged in the urine with an elimination half-life of approximately 8 hours.
Used in Animal Studies:
In animal studies, Balofloxacin is used to evaluate its safety and efficacy, showing no phototoxicity.
Originator
Chugai Pharmaceutical (Japan)
Manufacturing Process
(1) A mixture of ethyl 1-cyclopropyl-6,7,8-trifluoro-1,4-dihydro-4-
oxoquinoline-3-carboxylate (933 mg), 3-acetamidopiperidine (710 mg),
triethylamine (400 mg) and dimethylsulfoxide (10 ml) was heated at 100°C
for 2 hours with stirring. Thereafter the mixture was cooled down and ice
water was added thereto. The resulting mixture was extracted with chloroform
and the chloroform layer was washed with water three times before being
dried over anhydrous sodium sulfate. Removal of the solvent in vacuum
followed by purification by silica gel column chromatography (chloroformethanol)
gave ethyl 7-(3-acetamidopiperidin-1-yl)-1-cyclopropyl-6,8-difluoro-
1,4-dihydro-4-oxo quinoline-3-carboxylate (930 mg). Re-crystallization from
ethanol-ether afforded a colorless crystalline substance (MP: 217°-218°C).
(2) Ethyl 7-(3-acetamidopiperidin-1-yl)-1-cyclopropyl-6,8-difluoro-1,4-dihydro-
4-oxo-quinoline-3-carboxylate obtained from the foregoing step:
(a) (433 mg) of above product was dissolved in 6 N hydrochloric acid (5 ml)
and heated at 100°C. for 2.5 hours with stirring. After the removal of the
solvent in vacuum, methanol was added to the residue and the insoluble
materials were filtered off. Removal of the solvent followed by purification by
silica gel column chromatography (chloroform-methanol) gave hydrochloride
of 7-(3-aminopiperidin-1-yl)-1-cyclopropyl-6,8-difluoro-1,4-dihydro-4-
oxoquinoline-3-carboxylic acid (colorless, crystalline-powder). MP: color
change at about 272°C, decomposition at about 280°C.
(b) A mixture of 7-(3-acetamidopiperidin-1-yl)-1-cyclopropyl-6,8-difluoro-1,4-
dihydro-4-oxoquinoline-3-carboxylic acid (4.05 g), sodium methoxide (2.16 g)
and N,N-dimethylformamide (120 ml) was stirred for 2 hours at 100°-140°C.
The reaction mixture was concentrated in vacuum and water was added to the
residue. The mixture was neutralized with 1 N hydrochloric acid and the
neutralized mixture was then concentrated in vacuum. Purification of the
concentrated mixture by silica gel column chromatography (chloroformmethanol)
gave 7-(3-acetamidopiperidin-1-yl)-1-cyclopropyl-6-fluoro-1,4-
dihydro-8-methoxy-4-oxoquinoline-3-carboxylic acid. MP: 248°-250°C.
(c) 7-(3-Acetamidopiperidin-1-yl)-1-cyclopropyl-6-fluoro-1,4-dihydro-8-
methoxy-4-oxoquinoline-3-carboxylic acid (1.25 g) above obtained was
suspended in 6 N hydrochloric acid (30 ml) and ethanol (5 ml) and heated at
100°C for 3 hours. Then the reaction mixture was concentrated in vacuum
and the residue was purified by silica gel column chromatography
(chloroform:methanol:ammonium hydroxide=100:30:5) to afford 7-(3-
aminopiperidin-1-yl)-1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-4-
oxoquinoline-3-carboxylic acid. MP: 176°-177°C.
Therapeutic Function
Antibacterial
Pharmaceutical Applications
A 6-fluoro-8-methoxy quinolone derivative. It has good antistaphylococcal activity (MIC 0.4–4 mg/L), but is inactive against methicillin-resistant Staph. aureus (MRSA) and quinolone-resistant Staph. aureus; Str. pneumoniae is inhibited by 0.4 mg/L. It has good activity against Enterobacteriaceae, but is inactive against Ps. aeruginosa (MIC 8–16 mg/L). After a 200 mg oral dose a peak level of 1.7 mg/L is reached in 1 h. The apparent elimination halflife is about 8 h, rising to 13 h in elderly subjects. Plasma protein binding is about 16%. It was withdrawn from the market in Japan because of adverse events, but is available in China.
Check Digit Verification of cas no
The CAS Registry Mumber 127294-70-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,7,2,9 and 4 respectively; the second part has 2 digits, 7 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 127294-70:
(8*1)+(7*2)+(6*7)+(5*2)+(4*9)+(3*4)+(2*7)+(1*0)=136
136 % 10 = 6
So 127294-70-6 is a valid CAS Registry Number.
InChI:InChI=1/C20H24FN3O4/c1-22-11-4-3-7-23(9-11)17-15(21)8-13-16(19(17)28-2)24(12-5-6-12)10-14(18(13)25)20(26)27/h8,10-12,22H,3-7,9H2,1-2H3,(H,26,27)/t11-/m1/s1
