1281999-97-0Relevant academic research and scientific papers
Chemoenzymatic synthesis of α2-3-sialylated carbohydrate epitopes
Huang, Shengshu,Yu, Hai,Chen, Xi
experimental part, p. 117 - 128 (2011/12/16)
Sialic acids are common terminal carbohydrates on cell surface. Together with internal carbohydrate structures, they play important roles in many physiological and pathological processes. In order to obtain α2-3-sialylated oligosaccharides, a highly efficient one-pot three-enzyme synthetic approach was applied. The P. multocida-α2-3-sialyltransferase (PmST1) involved in the synthesis was a multifunctional enzyme with extremely flexible donor and acceptor substrate specificities. Sialyltransferase acceptors, including type 1 structure (Galβ1-3GlcNAcβProN 3), type 2 structures (Galβ1-4GlcNAcβProN3 and 6-sulfo-Galβ1-4GlcNAcβProN3), type 4 structure (Galβ1-3GalNAcβProN3), type 3 or core 1 structure (Galβ1-3GalNAcαProN3) and human milk oligoscaccharide or lipooligosaccharide lacto-N-tetraose (LNT) (Galβ1-3GlcNAcβ1- 3Galβ1-4GlcβProN3), were chemically synthesized. They were then used in one-pot three-enzyme reactions with sialic acid precursor ManNAc or ManNGc, to synthesize a library of naturally occurring α2-3-linked sialosides with different internal sugar structures. The sialylated oligosaccharides obtained are valuable probes for their biological studies.
