1283074-34-9Relevant articles and documents
Influenza virus H5N1 hemagglutinin (HA) T-cell epitope conjugates: Design, synthesis and immunogenicity
Skarlas, Theodore,Zevgiti, Stella,Droebner, Karoline,Panou-Pomonis, Eugenia,Planz, Oliver,Sakarellos-Daitsiotis, Maria
, p. 226 - 232 (2011)
The influenza virus, major surface glycoprotein hemagglutinin (HA) is one of the principal targets for the development of protective immunity. Aiming at contributing to the development of a vaccine that remains the first choice for prophylactic intervention, a reconstituted model of HA, mimicking its antigenic properties was designed, synthesized and tested in mice for the induction of protective immunity. Four helper T lymphocyte [HTL (T1, T3, T7 and T8)] and four cytotoxic lymphocyte [CTL (T2, T4, T5 and T6)] epitopes were coupled in two copies each to an artificial carrier, SOC4, which was formed by the repeating tripeptide Lys-Aib-Gly. The helical conformation of the SOC4-conjugates preserves the initial topology of the attached epitopes, which is critical for their immunogenic properties. Survival of immunized animals, ranged from 30 to 50%, points out the induction of protective immunity by using the SOC4-conjugates. A reconstituted mimic of the H5 HA antigen was formulated by chemoselective ligation of H5 HA T-cell epitopes to an artificial carrier SOC4 for evaluation of its immunogenicity. Copyright
