13080-39-2Relevant articles and documents
Engineering faster transglycosidases and their acceptor specificity
Tran, Linh T.,Blay, Vincent,Luang, Sukanya,Eurtivong, Chatchakorn,Choknud, Sunaree,González-Diáz, Humbert,Ketudat Cairns, James R.
supporting information, p. 2823 - 2836 (2019/06/13)
Transglycosidases are enzymes that have the potential to catalyze the synthesis of a wide range of high-value compounds starting from biomass-derived feedstocks. Improving their activity and broadening the substrate range are important goals to enable the widespread application of this family of biocatalysts. In this work, we engineered 20 mutants of the rice transglycosidase Os9BGlu31 and evaluated their catalysis in 462 reactions over 18 different substrates. This allowed us to identify mutants that expanded their substrate range and showed high activity, including W243L and W243N. We also developed double mutants that show very high activity on certain substrates and exceptional specificity towards hydrolysis, such as L241D/W243N. In order to guide a more general use of Os9BGlu31 variants as transglycosylation catalysts, we built cheminformatics models based on topological descriptors of the substrates. These models showed useful predictive potential on the external validation set and are allowing the identification of efficient catalytic routes to novel phytohormone and antibiotic glucoconjugates of interest.
Biotransformation of phenylcarboxylic acids by plant cell cultures
Ushiyama, Masashi,Kumagai, Shigehiro,Furuya, Tsutomu
, p. 3335 - 3339 (2007/10/02)
A suspension culture of Glycyrrhiza echinata converted benzoic acid into its glucosyl ester. Suspension cultures of Aconitum japonicum, Coffea arabica, Dioscoreophyllum cumminsii and Nicotiana tabacum, transformed benzoic acid into its gentiobiosyl ester in addition to the glucosyl ester. The suspension cultures of A.japonicum and G. echinata converted phenylacetic acid into the esters attached to the C-6 position of glucose, that is, 6-O-phenylacetyl-d-glucose and ethyl 6-O-phenylacetyl-β-d-glucopyranoside. That of D. cumminsii converted phenylacetic acid into the glucose ester and also into phenethyl β-d-glucopyranoside showing glucosylation after the reduction of the carboxylic group. These suspension cultures converted cinnamic acid into p-coumaric acid and its glucosyl ester and p-coumaric acid into its glucosyl ester. However, the conversion of caffeic acid was not observed. The suspension cultures of A.japonicum and C. arabica converted 3-phenylpropionic acid into its gentiobiosyl ester. On the other hand, the culture of D. cumminsii did not produce the glycosyl ester but instead 3-(4-hydroxyphenyl)propionic acid was formed, thus showing hydroxylation capability.
