1309581-49-4Relevant academic research and scientific papers
Genetically encoded copper-free click chemistry
Plass, Tilman,Milles, Sigrid,Koehler, Christine,Schultz, Carsten,Lemke, Edward A.
, p. 3878 - 3881 (2011/06/24)
A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).
