131347-76-7Relevant articles and documents
Improving Catalytic Activity and Reversing Enantio-Specificity of ω-Transaminase by Semi-Rational Engineering en Route to Chiral Bulky β-Amino Esters
Wang, Yingang,Feng, Jinhui,Dong, Wenyue,Chen, Xi,Yao, Peiyuan,Wu, Qiaqing,Zhu, Dunming
, p. 3396 - 3400 (2021)
The application of wild-type ω-transaminase was limited by steric hindrance towards bulky substrates, therefore improvement of the catalytic efficiency and stereoselectivity toward substrates with two bulky substituent adjacent to the carbonyl is of general interest. In this study, according to the double substrate binding pocket theory, a (S)-selective ω-transaminase from the Burkholderia vietnamiensis G4, which showed puny catalytic activity toward the β-keto esters with small steric hindrance, was engineered to accept bulky β-keto esters, which were not accessible by any wild-type enzyme. A few desired variants were obtained that exhibited activity toward bulky β-keto esters. Furthermore, a substrate-dependent shift in enantio-preference of HBV variant towards β-keto esters with linear or branched aliphatic substituents was observed. The best variant was applied to the asymmetric synthesis of aliphatic β-amino acids at semi-preparative scale with high yield and enantioselectivity. This study will improve the general understanding and inspire further engineering work for reversing enantio-specificity of ω-transaminases.
104. The enantioselective synthesis of β-amino acids, their α-hydroxy derivatives, and the N-terminal components of bestatin and microginin
Jefford, Charles W.,McNulty, James,Lu, Zhi-Hui,Wang, Jian Bo
, p. 1203 - 1216 (2007/10/03)
L-Aspartic acid by tosylation, anhydride formation, and reduction with NaBH4 was converted into (3S)-3-(tosylamino)butan-4-olide (8; Scheme 1). Treatment of 8 with ethanolic trimethylsilyl iodide gave the N-protected deoxy-iodo-β-homoserine ethyl ester 9. The latter, on successive nucleophilic displacement with lithium dialkylcuprates (→ 10a-e), alkaline hydrolysis (→ 11a-e), and reductive removal of the tosyl group, produced the corresponding 4-substituted (3R)-3-aminobutanoic acids 12a-e (ee >99%). Electrophilic hydroxylation of 8 (→ 19; Scheme 3), subsequent iodo-esterification (→ 21; Scheme 4), and nucleophilic alkylation and phenylation afforded, after saponification and deprotection, a series of 4-substituted (2S,3A)-3-amino-2-hydroxybutanoic acids 24 including the N-terminal acids 24e (= 3) and 24f (= 4) of bestatin and microginin (de >95%), respectively.
An enantiospecific synthesis of β-amino acids
Jefford,Wang
, p. 1111 - 1114 (2007/10/02)
L-Aspartic acid by regioselective modification of the α-carboxylic acid group, namely N-tosylation, anhydride formation, reduction, iodo-esterification, alkylation, and deprotection afforded a series of γ-alkyl β-aminobutyric acids of the R configuration (ee > 99%).
