1319744-64-3Relevant academic research and scientific papers
Synthesis of fluorescently labelled rhamnosides: Probes for the evaluation of rhamnogalacturonan II biosynthetic enzymes
Prifti, Efthymia,Goetz, Stephan,Nepogodiev, Sergey A.,Field, Robert A.
, p. 1617 - 1621 (2011)
Three fluorescently labelled saccharides 10-12, representing structures found in pectic glycan rhamnogalacturonan II (RG-II), were synthesised by chemical glycosylation of O-6 of diacetone-d-galactose followed by deprotection and reductive amination with amino-substituted fluorophore APTS. This convenient method installs a common aminogalactitol-based tether in order to preserve the integrity of the reducing end of specific carbohydrates of interest. APTS-labelled glycans prepared in this manner were purified by carbohydrate gel electrophoresis and subjected to capillary electrophoresis analysis, as a basis for the subsequent development of high sensitivity assays for RG-II-active enzymes.
