1320358-65-3Relevant academic research and scientific papers
Accelerated Protein Synthesis via One-Pot Ligation-Deselenization Chemistry
Mitchell, Nicholas J.,Sayers, Jessica,Kulkarni, Sameer S.,Clayton, Daniel,Goldys, Anna M.,Ripoll-Rozada, Jorge,Barbosa Pereira, Pedro José,Chan, Bun,Radom, Leo,Payne, Richard J.
supporting information, p. 703 - 715 (2017/05/15)
Peptide ligation chemistry has revolutionized protein science by facilitating access to synthetic proteins. Here, we describe the development of additive-free ligation-deselenization chemistry at β-selenoaspartate and γ-selenoglutamate that enables the generation of native polypeptide products on unprecedented timescales. The deselenization step is chemoselective in the presence of unprotected selenocysteine, which is highlighted in the synthesis of selenoprotein K. The power of the methodology is also showcased through the synthesis of three tick-derived thrombin-inhibiting proteins, each of which were assembled, purified, and isolated for biological assays within a few hours. The methodology described here should serve as a powerful means of accessing synthetic proteins, including therapeutic leads, in the future.
Chemoselective peptide ligation-desulfurization at aspartate
Thompson, Robert E.,Chan, Bun,Radom, Leo,Jolliffe, Katrina A.,Payne, Richard J.
supporting information, p. 9723 - 9727 (2013/09/23)
Asp-ecially useful: A synthetic β-mercapto aspartate residue facilitates the rapid ligation to a range of peptide thioesters. Following the ligation reaction (and without purification), chemoselective desulfurization of the β-mercapto moiety in the presence of unprotected cysteine residues afforded native peptide products. Copyright
Peptide ligations accelerated by N-terminal aspartate and glutamate residues
Thomas, Gemma L.,Hsieh, Yves S. Y.,Chun, Candy K. Y.,Cai, Zheng-Li,Reimers, Jeffrey R.,Payne, Richard J.
supporting information; experimental part, p. 4770 - 4773 (2011/11/12)
A novel application of intramolecular base catalysis confers enhanced reaction rates for aminolysis ligations between peptide thioesters and peptides bearing N-terminal aspartate or glutamate residues. The broad scope of this process and its application i
