1332848-71-1

Basic information

• Product Name: 5-[3-(Z-Gln-Gly-amido)-1-(E)-propenyl]-2'-deoxyuridine-5'-triphosphate
• CAS NO: 1332848-71-1
• Molecular Weight: 842.541
• Mol File: 1332848-71-1.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 5-[3-(Z-Gln-Gly-amido)-1-(E)-propenyl]-2'-deoxyuridine-5'-triphosphate(CAS DataBase Reference)
• NIST Chemistry Reference: 5-[3-(Z-Gln-Gly-amido)-1-(E)-propenyl]-2'-deoxyuridine-5'-triphosphate(1332848-71-1)
• EPA Substance Registry System: 5-[3-(Z-Gln-Gly-amido)-1-(E)-propenyl]-2'-deoxyuridine-5'-triphosphate(1332848-71-1)

Safety Data

• Hazardous Substances Data: 1332848-71-1(Hazardous Substances Data)

Upstream product

• 6610-42-0 α-N-carbobenzyloxy-L-glutamine-glycine 

Relevant articles and documents

Transglutaminase-mediated synthesis of a DNA-(Enzyme)n probe for highly sensitive DNA detection

A new synthetic strategy for DNA-enzyme conjugates with a novel architecture was explored using a natural cross-linking catalyst, microbial transglutaminase (MTG). A glutamine-donor substrate peptide of MTG was introduced at the 5-position on the pyrimidine of deoxyuridine triphosphate to prepare a DNA strand with multiple glutamine-donor sites by polymerase chain reaction (PCR). A substrate peptide that contained an MTG-reactive lysine residue was fused to the N terminus of a thermostable alkaline phoshatase from Pyrococcus furiosus (PfuAP) by genetic engineering. By combining enzymatically the substrate moieties of MTG introduced to the DNA template and the recombinant enzyme, a DNA-(enzyme)n conjugate with 1:n stoichiometry was successfully obtained. The enzyme/DNA ratio of the conjugate increased as the benzyloxycarbonyl-L-glutaminylglycine (Z-QG) moiety increased in the DNA template. The potential utility of the new conjugate decorated with signaling enzymes was validated in a dot blot hybridization assay. The DNA-(enzyme) n probe could clearly detect 104 copies of the target nucleic acid with the complementary sequence under harsh hybridization conditions, thereby enabling a simple detection procedure without cumbersome bound/free processes associated with a conventional hapten-antibody reaction-based DNA-detection system. DNA detector: A glutamine-modified DNA probe was synthesized by the polymerase chain reaction by using a glutamine-modified 2′-deoxyuridine 5′-triphosphate analogue as a substrate of DNA polymerase. The DNA-(alkaline phosphatase)n conjugate probe was highly sensitive and could directly visualize the target DNA bound on a membrane immediately after hybridization (see graphic).