1333382-75-4Relevant academic research and scientific papers
Biotechnological development of a practical synthesis of ethyl (S)-2-ethoxy-3-(p -methoxyphenyl)propanoate (EEHP): Over 100-fold productivity increase from yeast whole cells to recombinant isolated enzymes
Bechtold, Matthias,Brenna, Elisabetta,Femmer, Christian,Gatti, Francesco G.,Panke, Sven,Parmeggiani, Fabio,Sacchetti, Alessandro
, p. 269 - 276 (2012)
The coupling of the enantioselective reduction catalyzed by Old Yellow Enzymes (OYEs), together with the in situ substrate feeding product removal (SFPR) concept, significantly improved the productivity of the g-scale preparation of ethyl (S)-2-ethoxy-3-(p-methoxyphenyl)propanoate (EEHP), an important precursor of several PPAR-α/γ agonists, such as Tesaglitazar. The OYEs and the glucose dehydrogenase for cofactor regeneration were cloned, overexpressed in Escherichia coli, and purified. The synthetic sequence was completed by a NaClO2 oxidation employing cheap and environmentally friendly conditions. The product was obtained in 94% yield and with an ee of 98% over the two steps.
