1333432-86-2Relevant academic research and scientific papers
Seminaphthofluorescein-based fluorescent probes for imaging nitric oxide in live cells
Pluth, Michael D.,Chan, Maria R.,McQuade, Lindsey E.,Lippard, Stephen J.
, p. 9385 - 9392 (2011)
Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20-45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depend on the excitation wavelength; maximum fluorescence turn-on is achieved at excitations between 535 and 575 nm. The probes are highly selective for NO over other biologically relevant reactive nitrogen and oxygen species including NO3 -, NO2-, HNO, ONOO-, NO2, OCl-, and H2O2. The seminaphthofluorescein- based probes can be used to visualize endogenously produced NO in live cells, as demonstrated using Raw 264.7 macrophages.
