133502-31-5Relevant academic research and scientific papers
New Nucleoside Phosphoramidites and Coupling Protocols for Solid-Phase RNA Synthesis
Lyttle, Matthew H.,Wright, Peter B.,Sinha, Nanda D.,Bain, J. D.,Chamberlin, A. Richard
, p. 4608 - 4615 (1991)
The 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diethylphosphoramidites) 3, 5, 7, and 9, modified monomers for RNA synthesis, were prepared from 2-cyanoethyl N,N-diethylchlorophosphoramidite (1).In conjunction with newly developed coupling protocols for automated solid-phase synthesis, they afforded synthetic oligoribonucleotides up to 74 base units in length.The performance of the new compounds was compared to the analogous 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) 4, 6, 8, and 10.Complete removal of benzoyl groups from N2-benzoylguanosine, which was incorporated into some of the synthetic oligoribonucleotides, was demonstrated.Purification procedures by reverse phase HPLC and PAGE methods are also presented.
