1335100-09-8Relevant articles and documents
Novel water-soluble red-emitting poly(p -phenylenevinylene) derivative: Synthesis, characterization, and fluorescent acetylcholinesterase assays
Zhang, Wenjun,Zhu, Linna,Qin, Jingui,Yang, Chuluo
, p. 12059 - 12064 (2011/12/05)
A new cyano-substituted poly(p-phenylenevinylene) (PPV) derivative, MEOPS-CNPPV, is synthesized through Knoevenagel condensation of anionic diacetonitrile and neutral dialdehyde and characterized by 1H NMR, IR, elemental analysis, and gel-permeation chromatography (GPC). To our knowledge, the polymer is the first water-soluble red-emitting PPV derivative. The absorption and emission wavelength of this water-soluble conjugated polymer (CP) depend on the solvent. In buffer solution, the fluorescence of MEOPS-CNPPV is quenched by cationic dinitrobenzene derivatives. Further research indicates that dinitrobenzene derivative with a more flexible structure exhibits a larger Ksv. Making use of the charge reversal of dinitrobenzene-modified substrate, a "turn-on" method is developed for AChE activity assay with the new polymer as a fluorophore. This convenient and direct fluorometric assay thus provides a platform for novel red-emitting sensory systems.