133605-58-0Relevant academic research and scientific papers
N-chlorosuccinimide, an efficient peptide disulfide bond-forming reagent in aqueous solution
Postma, Tobias M.,Albericio, Fernando
, p. 14277 - 14280 (2013)
A novel method has been developed for the efficient formation of peptide disulfide bonds under aqueous conditions using N-chlorosuccinimide. Complete disulfide bond formation is achieved in 15 min with solvent mixtures containing water and acetonitrile. The Royal Society of Chemistry 2013.
Detection of Thiol Functionality and Disulfide Bond Formation by Polyoxometalate
Konno, Hiroyuki,Yasumiishi, Haruto,Aoki, Reika,Nitanai, Ikumi,Yano, Shigekazu
supporting information, p. 745 - 749 (2020/11/10)
The detection of thiol functionality and intramolecular disulfide bond formation of peptides using the α-Keggin type polyoxometalate molybdenum-oxygen cluster (H3PMo12O40·nH2O) is described. Our method entails the addition of this polyoxometalate to solutions of thiol, whereupon the color of the solution changes from colorless to deep blue. Reduction of the polyoxometalate from Mo(VI) to Mo(V) occurs with concomitant oxidation of the thiol functionality, to form disulfide bonds. To exemplify the utility this phenomenon, we accomplished the oxidation of glutathione, reduced linear oxytocin, bactenecin, and α-conotoxin SI; all of which proceeded smoothly and in good conversion in 24 h to less and were accomplished by a change in the color of the reaction solutions.
High-throughput production of two disulphide-bridge toxins
Upert, Grégory,Mourier, Gilles,Pastor, Alexandra,Verdenaud, Marion,Alili, Doria,Servent, Denis,Gilles, Nicolas
supporting information, p. 8408 - 8411 (2014/07/22)
A quick and efficient production method compatible with high-throughput screening was developed using 36 toxins belonging to four different families of two disulphide-bridge toxins. Final toxins were characterized using HPLC co-elution, CD and pharmacological studies. This journal is the Partner Organisations 2014.
N-Methyl-phenacyloxycarbamidomethyl (Pocam) group: A novel thiol protecting group for solid-phase peptide synthesis and peptide condensation reactions
Katayama, Hidekazu,Nakahara, Yoshiaki,Hojo, Hironobu
experimental part, p. 4653 - 4661 (2011/07/31)
In the so-called thioester method for the condensation of peptide segments, protecting groups for amino and thiol groups are required for chemoselective ligation. In this study, we developed a novel thiol protecting group, N-methyl-phenacyloxycarbamidomet
