133975-85-6 Usage
Description
5'-AMINO-MODIFIER-C 6-TFA CEP is a nucleoside phosphoramidite that plays a crucial role in the synthesis and modification of oligonucleotides. It features a 5'-amino modifier group, which facilitates the conjugation of functional molecules or labels to the oligonucleotide strand, enhancing its versatility in various applications. The C 6-TFA component indicates the presence of a trityl-protected 5'-azide, a common feature in solid-phase synthesis, which contributes to the stability and reactivity of the molecule. This chemical is widely utilized in molecular biology and biochemistry research for the modification and functionalization of nucleic acids, enabling a range of applications such as sequencing, PCR amplification, and imaging.
Uses
Used in Molecular Biology Research:
5'-AMINO-MODIFIER-C 6-TFA CEP is used as a key component in the synthesis of modified oligonucleotides for various molecular biology applications. Its 5'-amino modifier group allows for the attachment of functional molecules or labels, enhancing the capabilities of the oligonucleotides in research and diagnostics.
Used in Biochemistry Research:
In biochemistry, 5'-AMINO-MODIFIER-C 6-TFA CEP is employed as a versatile building block for the creation of customized oligonucleotides. The trityl-protected 5'-azide provides stability during solid-phase synthesis, ensuring the production of high-quality modified nucleic acids for use in various biochemical assays and analyses.
Used in Sequencing Applications:
5'-AMINO-MODIFIER-C 6-TFA CEP is used as a modifier in sequencing processes to improve the accuracy and efficiency of nucleic acid analysis. The ability to attach specific labels or functional groups to the oligonucleotide strand allows for enhanced detection and identification of nucleotide sequences.
Used in PCR Amplification:
5'-AMINO-MODIFIER-C 6-TFA CEP is utilized in the design of primers for PCR amplification, where the 5'-amino modifier group can be used to attach fluorescent labels or other detection molecules. This modification aids in the visualization and quantification of PCR products, improving the sensitivity and specificity of the amplification process.
Used in Imaging Techniques:
In imaging applications, 5'-AMINO-MODIFIER-C 6-TFA CEP is employed to create oligonucleotide probes that can be conjugated with imaging agents, such as fluorescent dyes or radionuclides. These modified probes enable the visualization of specific nucleic acid sequences within biological samples, providing valuable insights into gene expression, localization, and interaction.
Overall, 5'-AMINO-MODIFIER-C 6-TFA CEP is a versatile and essential chemical in the fields of molecular biology and biochemistry, offering a wide range of applications in research, diagnostics, and therapeutic development. Its unique properties and functionalities make it an indispensable tool for the synthesis and modification of oligonucleotides, driving advancements in our understanding of nucleic acid biology and its applications.
Check Digit Verification of cas no
The CAS Registry Mumber 133975-85-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,3,9,7 and 5 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 133975-85:
(8*1)+(7*3)+(6*3)+(5*9)+(4*7)+(3*5)+(2*8)+(1*5)=156
156 % 10 = 6
So 133975-85-6 is a valid CAS Registry Number.
