134009-25-9Relevant academic research and scientific papers
Design, synthesis and systematic evaluation of all possible cyclic dinucleotides (CDNs) that activate human stimulator of interferon genes (STING) variants
Li, Lu-Yuan,Ma, De-Jun,Wang, Chuan-Lin,Wang, Da-Wei,Wang, Zheng-Hua,Wen, Xin,Xi, Zhen,Zhang, Hang,Zhang, Qiang-Zhe,Zhao, Can-Can
, (2020/04/10)
Cyclic dinucleotides (CDNs) are known to activate stimulator of interferon genes (STING) and induce type I interferon responses, therefor possess great potentials to be of immunotherapeutic value for cancers and infectious diseases. However, the existence
Spectroscopic and enzymatic chracterization of 2'-5' and 3'-5' RNA hexamers AACCUU synthesised by phosphotriester approach in solution using 2'-t-butyldimethylsilyl protection
Gopalakrishnan,Ganesh,Gunjal,Likhite
, p. 1075 - 1090 (2007/10/02)
Comparative 1H, 31P NMR and CD spectroscopic results and enzymatic cleavage of single stranded RNA hexamers which have identical base sequence (AACCUU) but are regioisomeric at the internucleotide phosphate linkages (2'-5', 3'-5' and their covalent hybrid) are presented. The 31P NMR results revealed significant differences in local phosphate backbone conformation among these isomers, with 2'-5' isomer exhibitingf maximum heterogeneity as compared to the 3'-5' isomer, the analogous DNA hexamer and the covalent hybrid hexamer. In contrast to this, there are no appreciable differences in the overall base-base stacking as seen in the CD spectra of 2'-5' and 3'-5' isomers. All RNA hexamers were synthesised by solution phase phosphotriester chemistry with t-butyldimethylsilyl (TBDMS) as 2'-O-protecting group. The fully protected oligorobomers were deprotected in two steps: (i) saturated anhydrous MeOH-NH3 for phosphate and amino deprotections and (ii) TBAF for removal of 2'-O-TBDMS group. Use of sat. MeOH-NH3 (instead of aq. NH3) prevents 2'-3' internucleotide phosphate migrations, chain fragmentations and 5'-terminal modifications by neighbouring group participation (NGP). The retention of isomeric integrity and absence of 5'-terminal modification in the final products was established by digestion with several nucleases.
