134031-86-0 Usage
Uses
Used in Oligonucleotide Synthesis:
5'-O-[(N,N-DIISOPROPYLAMINO)-(2-CYANOETHOXY)PHOSPHINYL]-3'-O-(4,4'-DIMETHOXYTRITYL)-THYMIDINE is used as a building block for the synthesis of oligonucleotides. It is particularly useful for incorporating an unmodified reverse dT nucleobase within an oligonucleotide sequence, which can be essential for specific applications in molecular biology and biotechnology.
Used in Research and Development:
In the field of research and development, 5'-O-[(N,N-DIISOPROPYLAMINO)-(2-CYANOETHOXY)PHOSPHINYL]-3'-O-(4,4'-DIMETHOXYTRITYL)-THYMIDINE is used as a key component in the design and synthesis of modified oligonucleotides. These modified oligonucleotides can be employed in various research applications, such as gene expression studies, gene regulation, and the investigation of molecular interactions.
Used in Diagnostics:
5'-O-[(N,N-DIISOPROPYLAMINO)-(2-CYANOETHOXY)PHOSPHINYL]-3'-O-(4,4'-DIMETHOXYTRITYL)-THYMIDINE is also used in the development of diagnostic tools, such as PCR primers and DNA probes. These tools are essential for the detection and identification of specific genetic sequences, which can be crucial in the diagnosis of various diseases and conditions.
Used in Therapeutics:
In the therapeutics industry, 5'-O-[(N,N-DIISOPROPYLAMINO)-(2-CYANOETHOXY)PHOSPHINYL]-3'-O-(4,4'-DIMETHOXYTRITYL)-THYMIDINE can be used in the development of antisense oligonucleotides and other gene therapy approaches. These modified oligonucleotides can be designed to target specific genes or RNA molecules, potentially leading to novel treatments for various diseases, including cancer and genetic disorders.
Check Digit Verification of cas no
The CAS Registry Mumber 134031-86-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,4,0,3 and 1 respectively; the second part has 2 digits, 8 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 134031-86:
(8*1)+(7*3)+(6*4)+(5*0)+(4*3)+(3*1)+(2*8)+(1*6)=90
90 % 10 = 0
So 134031-86-0 is a valid CAS Registry Number.
134031-86-0Relevant academic research and scientific papers
Nucleotides. Part LXV. Synthesis of 2'-Deoxyribonucleoside 5'-Phosphoramidites: New Building Blocks for the Inverse (5'-3')-Oligonucleotide Approach
Wagner, Thomas,Pfleiderer, Wolfgang
, p. 2023 - 2035 (2007/10/03)
The syntheses of the 3'-O-(4,4'-dimethoxytrityl)-protected 5'-phosphoramidites 25-28 and 5'-(hydrogen succinates) 29-32, which can be used as monomeric building blocks for the inverse (5'-3')-oligodeoxyribonucleotide synthesis are described (Scheme). These activated nucleosides and nucleotides were obtained by two slightly different four-step syntheses starting with the base-protected nucleosides 13-20. For the protection of the aglycon residues, the well-established 2-(4-nitrophenyl)ethyl (npe) and [2-(4-nitrophenyl)ethoxy]carbonyl (npeoc) groups were used. The assembly of the oligonucleotides required a slightly increased coupling time of 3 min in application of the common protocol (see Table 1). The use of pyridinium hydrochloride as an activator (instead of 1H-tetrazole) resulted in an extremely shorter activation time of 30 seconds. We established the efficiency of this inverse strategy by the synthesis of the oligonucleotide 3'-conjugates 33 and 34 which carry lipophilic caps derived from cholesterol and vitamin E, respectively, as well as by the formation of (3'-3')- and (5'-5')-internucleotide linkages (see Table 2).
An inverse approach in oligodeoxyribonucleotide synthesis
Wagner, Thomas,Pfleiderer, Wolfgang
, p. 1657 - 1660 (2007/10/03)
We synthesized 3'-O-dimetboxytrityl-5'O-phosphoramidites and 5'-O- succinates which can be used as monomeric building blocks for the built up of oligodeoxyribonucleotides in the alternative 5'-3' direction. With this inverse strategy oligonucleotide 3'-conjugates as well as 3'-3' and 5'-5' internucleotidic linkages can be easily formed.
