FLUORESCEIN-12-UTP is a fluorescently labeled nucleotide that serves as a substrate for RNA polymerases in in vitro transcription reactions. It is a derivative of uridine triphosphate (UTP) with a fluorescein molecule attached, allowing for the incorporation of a fluorescent tag into RNA transcripts. This modification enables the detection and analysis of RNA molecules through various techniques, such as in situ hybridization, direct fluorescence detection, and ELISA.
134367-01-4
Post Buying Request-
-
Basic information
- Product Name: FLUORESCEIN-12-UTP
- Synonyms: FLUORESCEIN-12-UTP;CHROMATIDE(TM) FLUORESCEIN-12-UTP
- CAS NO:134367-01-4
- Molecular Formula: C38H41N4O20P3
- Molecular Weight: 966.67
- EINECS: N/A
- Product Categories: N/A
- Mol File: 134367-01-4.mol
-
Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: -20°C
- Solubility: N/A
- CAS DataBase Reference: FLUORESCEIN-12-UTP(CAS DataBase Reference)
- NIST Chemistry Reference: FLUORESCEIN-12-UTP(134367-01-4)
- EPA Substance Registry System: FLUORESCEIN-12-UTP(134367-01-4)
-
Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany:
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 134367-01-4(Hazardous Substances Data)
134367-01-4 Suppliers
Recommended suppliers
134367-01-4 Usage
Uses
Used in Molecular Biology Research:FLUORESCEIN-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases in in vitro transcription reactions. It replaces UTP, allowing for the incorporation of the fluorescent label into the synthesized RNA molecules.
Used in RNA Detection and Analysis:
Labeled RNA produced using FLUORESCEIN-12-UTP can be detected and analyzed through various methods, such as in situ hybridization, which allows for the visualization of specific RNA sequences within cells or tissues. This technique is particularly useful for studying gene expression patterns and understanding the spatial distribution of RNA molecules.
Used in Fluorescence Detection:
The fluorescent label on RNA transcripts generated using FLUORESCEIN-12-UTP enables direct fluorescence detection. This method allows for the rapid and sensitive detection of specific RNA sequences, making it a valuable tool for various molecular biology applications, including diagnostics and therapeutics development.
Used in Enzyme-Linked Immunosorbent Assay (ELISA):
Labeled RNA can also be detected using ELISA with Anti-Fluorescein-AP (Alkaline Phosphatase) or Anti-Fluorescein-POD (Peroxidase) Fab fragments. This technique provides a highly sensitive and quantitative method for detecting specific RNA sequences, making it useful for research and diagnostic applications.
Quality:
The typical analysis of FLUORESCEIN-12-UTP is at least 85% purity, as determined by high-performance liquid chromatography (HPLC), area percentage. This ensures that the product is of high quality and suitable for use in various molecular biology applications.
Check Digit Verification of cas no
The CAS Registry Mumber 134367-01-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,4,3,6 and 7 respectively; the second part has 2 digits, 0 and 1 respectively.Calculate Digit Verification of CAS Registry Number 134367-01:
(8*1)+(7*3)+(6*4)+(5*3)+(4*6)+(3*7)+(2*0)+(1*1)=114
114 % 10 = 4
So 134367-01-4 is a valid CAS Registry Number.
134367-01-4Upstream product
134367-01-4Downstream Products
Post a RFQ
1
What can I do for you?
Get Best Price
Get Best Price for 134367-01-4
FLUORESCEIN-12-UTP
