1353994-06-5Relevant articles and documents
Thermosensitive peptide-hybrid ABC block copolymers obtained by ATRP: Synthesis, self-assembly, and enzymatic degradation
De Graaf, Albert J.,Mastrobattista, Enrico,Vermonden, Tina,Van Nostrum, Cornelus F.,Rijkers, Dirk T.S.,Liskamp, Rob M.J.,Hennink, Wim E.
, p. 842 - 851 (2012)
Peptide-hybrid ABC block copolymers were synthesized by growing two different polymer chains from a native peptide using atom transfer radical polymerization (ATRP). To this end, two different ATRP initiators were coupled via orthogonal methods to the N- and C-terminus of the peptide Ser-Gly-Pro-Gln-Gly-Ile-Phe-Gly-Gln-Met-Gly, a substrate for matrix metalloproteases 2 and 9. First, a hydrophilic block of poly(oligo(ethylene glycol) methyl ether methacrylate) (pOEGMA) was polymerized from the peptide's C-terminus. Before polymerization of the second block, the first living chain end was inactivated by substitution of its Cl-terminus with azide under mild conditions. Then, a thermosensitive block of poly(N-isopropylacrylamide) (pNIPAm) was polymerized from the peptide's N-terminus. Well-defined polymers were obtained with good control over both block sizes. The resulting polymers self-assembled into micelles above the cloud point of the pNIPAm block. As anticipated, it was shown that the peptide linkage between the polymer blocks can be cut by a metalloprotease, leading to "shedding" of the corona of the micelles which makes these systems potentially suitable for enzyme-triggered drug delivery.
