1356820-99-9Relevant academic research and scientific papers
Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents
Seio, Kohji,Kurohagi, Sayako,Kodama, Erika,Masaki, Yoshiaki,Tsunoda, Hirosuke,Ohkubo, Akihiro,Sekine, Mitsuo
, p. 994 - 1006 (2012/04/10)
We have developed new artificial oligonucleotides which distinguish short RNA targets from long ones. The modification of the 5′ termini of oligonucleotides by using adenosine derivatives that possess a bulky cyclohexyl phosphate moiety at their base moiety and a phosphate group at the position of their 5′-hydroxyl group maximized their short RNA selectivity. The 2′-O-methyl-RNA (5′-XCmAmAmC mCmUmAmCmUm) having these modifications exhibits ca. 10 °C higher Tm in the duplexes with the complementary short RNA (3′-GUUGGAUGA-5′) than with the long RNA (3′-AUUAUAUGUUGGAUGAUGGUUA-5′). The oligodeoxynucleotides having the same modification exhibited similar selectivity. Such short-RNA selective binding of terminally modified oligonucleotides can be employed to distinguish between mature microRNAs and pre-microRNAs.
