135788-07-7 Usage
Uses
Used in Biochemical Research:
2,3-BIS(3-CHLOROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE is used as a substrate in assays for the quantification of viable cells, allowing researchers to measure cellular metabolic activity and viability.
Used in Cell Proliferation Studies:
In the field of cell biology, 2,3-BIS(3-CHLOROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE is used as an indicator in cell proliferation assays, helping to determine the growth rate and population dynamics of cells.
Used in Cytotoxicity Assessments:
2,3-BIS(3-CHLOROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE is employed as a tool in cytotoxicity studies to evaluate the effects of various substances on cell viability, providing insights into potential toxic effects and mechanisms of action.
Used in Drug Screening:
In pharmaceutical research, 2,3-BIS(3-CHLOROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE is used in drug screening processes to test the efficacy and safety of new compounds by assessing their impact on cell viability and metabolic activity.
Used in Toxicology:
2,3-BIS(3-CHLOROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE is utilized in toxicological studies to identify and measure the toxic effects of chemicals, environmental factors, or biological agents on cells, contributing to the understanding of toxicity mechanisms and risk assessment.
Check Digit Verification of cas no
The CAS Registry Mumber 135788-07-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,5,7,8 and 8 respectively; the second part has 2 digits, 0 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 135788-07:
(8*1)+(7*3)+(6*5)+(5*7)+(4*8)+(3*8)+(2*0)+(1*7)=157
157 % 10 = 7
So 135788-07-7 is a valid CAS Registry Number.
135788-07-7Relevant articles and documents
Anti-Plasmodium activity of tetrazolium salts
Cui, Xilin,Vlahakis, Jason Z.,Crandall, Ian E.,Szarek, Walter A.
, p. 1927 - 1947 (2008/09/21)
We have previously reported that sulfated cyclodextrins inhibit the invasion of Plasmodium merozoites by interacting with receptors present on the surface of erythrocytes. The observation that tetrazolium salts formed stable complexes with the inhibitory sulfated cyclodextrins suggested that tetrazolium salts might have anti-Plasmodium activity as well. Evaluation of commercially available tetrazolium salts indicated that some were active in the low nanomolar range and showed specificity in their inhibition of Plasmodium. Synthesis of a further 54 structures allowed us to determine that activity results from an aromatic component attached to the tetrazolium carbon atom (R1) and its size is not critical to the activity of the compound. Nitro modifications of active compounds are poorly tolerated, however, the presence of halogen atoms on aromatic groups attached to the nitrogen atoms of the tetrazolium ring (R2 and R3) has little effect on activity. Methoxy groups are tolerated on R2 and R3 components; however, they are disruptive on the R1 component. The overall results suggest that the R1 component is interacting with a specific hydrophobic environment and the R2 and R3 components are less constrained. The activity of these compounds in several human and mouse Plasmodium cultures suggests that the compounds interact with a component of the parasite that is both essential and conserved.
