1365543-40-3

Upstream product

• 391903-19-8 N-methoxy N-methyl 3,4-dinitrobenzoic acid amide 
• 619-66-9 4-Carboxybenzaldehyde 
• 660432-02-0 N-methoxy N-methyl 3,4-diaminobenzoic acid amide 
• 24376-18-9 3,4-dinitrobenzoyl chloride 
• 528-45-0 3,4-dinitrobenzoic acid 

Downstream Products

• 1365543-42-5 4-[{4-(5-formylbenzimidazol-2-yl)benzoyl}amino]-2,2,6,6-tetramethylpiperidin-1-oxyl 
• 1365543-31-2 4-(4-{5-[5-(4-methylpiperazin-1-yl)benzimidazol-2-yl]benzimidazol-2-yl}benzoylamino)-2,2,6,6-tetramethylpiperidin-1-oxyl 
• 1365543-32-3 4-[(4-{5-[5-({[(2,2,6,6-tetramethylpiperidin-1-oxyl)-4-yl]amino}carbonyl)benzimidazol-2-yl]benzimidazol-2-yl}benzoyl)amino]-2,2,6,6-tetramethylpiperidin-1-oxyl 
• 1365543-41-4 4-{[4-(5-{[(1-methoxy-1-methyl)amino]carbonyl}benzimidazol-2-yl)benzoyl]amino}-2,2,6,6-tetramethylpiperidin-1-oxyl 

Relevant academic research and scientific papers

Novel bisbenzimide-nitroxides for nuclear redox imaging in living cells

Nuclear oxidative stress damages genomic DNA and may lead to cell death, leading to aging and aging-related disorders. Though it is important to measure the nuclear oxidative stress separately, there are still little examples that applicable to living cells. We have designed and synthesized three bisbenzimide-nitroxides as probes to selectively visualize nuclear redox changes in terms of fluorescence. Compound 3, containing two radical moieties, showed the largest reduction-induced fluorescence change, with good localization in nuclei. RAW264.7 murine macrophage cells were loaded with compound 3 and then treated with 100 μM hydrogen peroxide for 5 min to show the fluorescence increase. This fluorescence increase was inhibited by pretreatment of 1 mM ascorbic acid. These results show that compound 3 was suitable for nuclear-specific redox imaging in murine macrophages.