136632-31-0

Basic information

• Product Name: 5-(N-(N-biotinyl-epsilon-aminocaproyl-*gamma-amin
• Synonyms: Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
• CAS NO: 136632-31-0
• Molecular Formula: C₃₂H₅₂N₇O₁₈P₃S
• Molecular Weight: 947.78
• Mol File: 136632-31-0.mol
• Article Data: 0

Chemical Properties

• Storage Temp.: -20°C
• CAS DataBase Reference: 5-(N-(N-biotinyl-epsilon-aminocaproyl-*gamma-amin(CAS DataBase Reference)
• NIST Chemistry Reference: 5-(N-(N-biotinyl-epsilon-aminocaproyl-*gamma-amin(136632-31-0)
• EPA Substance Registry System: 5-(N-(N-biotinyl-epsilon-aminocaproyl-*gamma-amin(136632-31-0)

Safety Data

• Hazardous Substances Data: 136632-31-0(Hazardous Substances Data)

Usage

Uses

For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.Biotin-16-dUTP is used as asubstrate for: Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)Taq DNA polymeraseReverse transcriptase (e.g., Transcriptor)Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrateBiotin Luminescence Detection Kit

General Description

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.

Biochem/physiol Actions

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.