1374221-64-3Relevant academic research and scientific papers
Butyl pocket formation in the vitamin D receptor strongly affects the agonistic or antagonistic behavior of ligands
Yoshimoto, Nobuko,Sakamaki, Yuta,Haeta, Minoru,Kato, Akira,Inaba, Yuka,Itoh, Toshimasa,Nakabayashi, Makoto,Ito, Nobutoshi,Yamamoto, Keiko
, p. 4373 - 4381 (2012)
Previously, we reported that 22S-butyl-25,26,27-trinor-1α24- dihydroxyvitamin D32 represents a new class of antagonist for the vitamin D receptor (VDR). The crystal structure of the ligand-binding domain (LBD) of VDR complexed with 2 showed the formation of a butyl pocket to accommodate the 22-butyl group and insufficient interactions between ligand 2 and the C-terminus of VDR. Here, we designed and synthesized new analogues 5a-c and evaluated their biological activities to probe whether agonistic activity is recovered when the analogue restores interactions with the C-terminus of VDR. Analogues 5a-c exhibited full agonistic activity in transactivation. Interestingly, 5c, which bears a 24-diethyl group, completely recovered agonistic activity, although 3c and 4c act as an antagonist and a weak agonist, respectively. The crystal structures of VDR-LBD complexed with 3a, 4a, 5a, and 5c were solved, and the results confirmed that butyl pocket formation in VDR strongly affects the agonistic or antagonistic behaviors of ligands.
