1375482-36-2Relevant academic research and scientific papers
NANOPARTICLE, LIPOSOMES, POLYMERS, AGENTS AND PROTEINS MODIFIED WITH REVERSIBLE LINKERS
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Page/Page column 81, (2012/09/10)
Pharmaceutical, chemical and biological agents containing a reversible disulfide linker are described. These agents can also be covalently bound or contained in delivery vehicles for delivering the agents to desired targets or areas. Also described are delivery vehicles which contain an agent having a reversible disulfide linker and to vehicles that are covalently linked to the agent containing a reversible disulfide linker. The modifications described herein can modify properties of the agents and vehicles, thereby providing desired solubility, stability, hydrophobicity and targeting while the reversibility of the linker can leave the agent to which it is attached free from residual chemical groups after being reduced.
Rendering protein-based particles transiently insoluble for therapeutic applications
Xu, Jing,Wang, Jin,Luft, J. Christopher,Tian, Shaomin,Owens, Gary,Pandya, Ashish A.,Berglund, Peter,Pohlhaus, Patrick,Maynor, Benjamin W.,Smith, Jonathan,Hubby, Bolyn,Napier, Mary E.,Desimone, Joseph M.
supporting information; experimental part, p. 8774 - 8777 (2012/07/02)
Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker leaves no chemical residue on the reactive amino group. Delivery of a self-replicating RNA was achieved via the transiently insoluble PRINT protein particles. These protein particles can provide new opportunities for drug and gene delivery.
