138038-96-7Relevant academic research and scientific papers
Methods and compositions for protein labeling using lipoic acid ligases
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Sheet 15, (2016/04/05)
The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.
Computational design of a red fluorophore ligase for site-specific protein labeling in living cells
Liu, Daniel S.,Nivón, Lucas G.,Richter, Florian,Goldman, Peter J.,Deerinck, Thomas J.,Yao, Jennifer Z.,Richardson, Douglas,Phipps, William S.,Ye, Anne Z.,Ellisman, Mark H.,Drennan, Catherine L.,Baker, David,Ting, Alice Y.
, p. E4551 - E4559 (2015/02/19)
Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.
Highly reactive trialkylsilylation reagents derived from bis(trifluoromethanesulfonyl)imide - Silylation of functional groups, alkines and reactive aromatics
Simchen, Gerhard,Jonas, Simon
experimental part, p. 506 - 512 (2011/10/17)
The synthesis of trialkylsilyl-bis(trifluormethanesulfonyl)imides 3 is described. Compounds 3 with bulky trialkylsilyl groups 3a,b only exist in the silatautomeric form 3a′,b′ under usual conditions, 3c in the N-trimethylsilyl structure. Despite of the bulky silyl substituents in 3a′,b′ their reactivity is higher than that of trimethylsilyltriflate. Alcohols, carbonyl compounds, nitroalkanes and carboxylic acid esters are silylated in good yields, especially by the more reactive triisopropylsilyl derivative 3b′ in presence of tertiary amines, tert.-Butyl carboxylates and benzylcarboxylates are cleaved. Monosubstituted alkines and electron-rich (hetero) aromatics are carbosilylated in presence of N-ethyl-diisopropylamine.
Transition Metal Directed Synthesis of Moracin M, a Phytoalexin of Morus alba Linn.
Mann, Inderjit S.,Widdowson, David A.,Clough, John M.
, p. 7981 - 7990 (2007/10/02)
2-(5-Resorcinyl)benzofurans have been synthesised by the palladium catalysed cross coupling of 2-trimethylstannyl- or 2-bromozinc benzofurans with the appropriately functionalised 5-iodoresorcinols.These were synthesised by the tri-isopropylsilyloxy direc
