1388771-64-9Relevant academic research and scientific papers
Polymerase incorporation of pyrene-nucleoside triphosphates
Hollenstein, Marcel,Wojciechowski, Filip,Leumann, Christian J.
supporting information; experimental part, p. 4428 - 4430 (2012/08/28)
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.
