139338-71-9Relevant academic research and scientific papers
Spacer molecules in peptide sequences: Incorporation into analogues of atrial natriuretic factor
Boumrah, Derradji,Campbell, Malcolm M.,Fenner, Simon,Kinsman, Richard G.
, p. 6977 - 6992 (2007/10/03)
In the present study, 10 modified human atrial natriuretic factor (hANF) analogues were designed using solid phase synthesis. This was carried out by replacing 'alkyl or glycol' spacer with octapeptide sequence within die cyclic portion of hANF in each annlogue synthesised. The unnatural amino acid spacers (1b) and (2d) have been synthesised using solution chemistry. The latter spacers were successfully incorporated into the peptide structure, using solid phase synthesis assembly. Amongst the ten analogues, thus prepared, two in which the alkyl spacer was used to substitute amino acid residues Gly15 to Gly21 and Arg14 to Leu21 to give 4a and 4b, successively. In the purification process of the latter analogues (4a, 4b), problems of their severe solubility were encountered. The eight glycol-spaced analogues (5a-5h) were successfully synthesised and purified using HPLC. The structure of (5a-5h) was confirmed by the presence of mass ion peaks in the atom bombardment mass spectroscopy (FAB MS) and by NMR. The latter analogues were tested, in vivo, for their ability to bind to specific hANF receptors, as agonists or antagonists. The biological results have showed that none of these analogues (5a-5h) were active.
Spacer molecules in peptide sequences: Incorporation into analogues of atrial natriuretic factor
Boumrah, Deradji,Campbell, Malcolm M.,Fenner, Simon,Kinsman, Richard G.
, p. 7735 - 7738 (2007/10/02)
The spacer reagents FmocHN(CH2CH2CH2)3CH 2COOPfp (1b) and FmocHN(CH2CH2O) 3CH2COOPfp (2d) have been prepared and used to substitute for tetra-residue sequences in the cyclic portion of Atrial Natriuretic Factor (ANF) by solid phase peptide assembly.
