1394003-19-0Relevant academic research and scientific papers
Red fluorescent scaffold for highly sensitive protease activity probes
Kushida, Yu,Hanaoka, Kenjiro,Komatsu, Toru,Terai, Takuya,Ueno, Tasuku,Yoshida, Kengo,Uchiyama, Masanobu,Nagano, Tetsuo
supporting information; experimental part, p. 3908 - 3911 (2012/07/03)
We have developed a novel red fluorescent dye, 2Me SiR600 (λem = 613 nm), in which the O atom of Rhodamine Green at the 10 position of the xanthene moiety is replaced with a Si atom, as a scaffold for probes to detect protease activity with extremely high S/N ratio. As proof of concept, we designed and synthesized probes for caspase-3 activity (Z-DEVD-SiR600) and leucine aminopeptidase activity (Leu-SiR600). Caspase-3-mediated cleavage of Z-DEVD-SiR600 resulted in a large bathochromic shift (93 nm) of the absorption maximum and a 432-fold fluorescence enhancement.
