1397690-17-3Relevant academic research and scientific papers
Fluorogenic assay of alkaline phosphatase activity based on the modulation of excited-state intramolecular proton transfer
Park, Jihye,Helal, Aasif,Kim, Hong-Seok,Kim, Youngmi
, p. 5541 - 5544 (2012)
A new fluorogenic substrate 1, which enables the fast and quantitative analysis of alkaline phosphatase activity, has been developed. Selective enzymatic hydrolysis of 1 instantly generated fluorescent compound 2 in aqueous media, which undergo an excited-state intramolecular proton transfer process, resulting in a remarkable fluorescence turn-on signal with an unusually large Stokes shift.
