1399581-68-0Relevant academic research and scientific papers
Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
Miyano, Yuka,Tsukuda, Senko,Sakimoto, Ippei,Takeuchi, Ryo,Shimura, Satomi,Takahashi, Noriyuki,Kusayanagi, Tomoe,Takakusagi, Yoichi,Okado, Mami,Matsumoto, Yuki,Takakusagi, Kaori,Takeuchi, Toshifumi,Kamisuki, Shinji,Nakazaki, Atsuo,Ohta, Keisuke,Miura, Masahiko,Kuramochi, Kouji,Mizushina, Yoshiyuki,Kobayashi, Susumu,Sugawara, Fumio,Sakaguchi, Kengo
, p. 3985 - 3990 (2012)
Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.
