1401341-45-4Relevant articles and documents
Phenyl glyoxal probes
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Page/Page column 23; 24; 28; 29; 35; 36, (2016/06/13)
Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described
Seeing citrulline: Development of a phenylglyoxal-based probe to visualize protein citrullination
Bicker, Kevin L.,Subramanian, Venkataraman,Chumanevich, Alexander A.,Hofseth, Lorne J.,Thompson, Paul R.
supporting information, p. 17015 - 17018 (2013/01/15)
Protein arginine deiminases (PADs) catalyze the hydrolysis of peptidyl arginine to form peptidyl citrulline. Abnormally high PAD activity is observed in a host of human diseases, but the exact role of protein citrullination in these diseases and the identities of specific citrullinated disease biomarkers remain unknown, largely because of the lack of readily available chemical probes to detect protein citrullination. For this reason, we developed a citrulline-specific chemical probe, rhodamine-phenylglyoxal (Rh-PG), which we show can be used to investigate protein citrullination. This methodology is superior to existing techniques because it possesses higher throughput and excellent sensitivity. Additionally, we demonstrate that this probe can be used to determine the kinetic parameters for a number of protein substrates, monitor drug efficacy, and identify disease biomarkers in an animal model of ulcerative colitis that displays aberrantly increased PAD activity.
