1401413-26-0Relevant academic research and scientific papers
A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans
Wang, Zhen,Chinoy, Zoeisha S.,Ambre, Shailesh G.,Peng, Wenjie,McBride, Ryan,De Vries, Robert P.,Glushka, John,Paulson, James C.,Boons, Geert-Jan
, p. 379 - 383 (2013/08/23)
A systematic, efficient means of producing diverse libraries of asymmetrically branched N-glycans is needed to investigate the specificities and biology of glycan-binding proteins. To that end, we describe a core pentasaccharide that at potential branching positions is modified by orthogonal protecting groups to allow selective attachment of specific saccharide moieties by chemical glycosylation. The appendages were selected so that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans. The power of the methodology was demonstrated by the preparation of a series of complex oligosaccharides that were printed as microarrays and screened for binding to lectins and influenza-virus hemagglutinins, which showed that recognition is modulated by presentation of minimal epitopes in the context of complex N-glycans.
COMPOUNDS AND METHODS FOR CHEMICAL AND CHEMO-ENZYMATIC SYNTHESIS OF COMPLEX GLYCANS
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Page/Page column 53-54, (2012/10/18)
The present invention provides chemical and chemo-enzymatic methods for the synthesis of a wide array of complex asymmetric multi-antennary glycans.
