1402691-99-9Relevant academic research and scientific papers
Sensitive near-infrared fluorescent probes for thiols based on Se-N bond cleavage: Imaging in living cells and tissues
Wang, Rui,Chen, Lingxin,Liu, Ping,Wang, Yunqing,Zhang, Qin
, p. 11343 - 11349,7 (2012)
Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues. Turn-on fluorescent probes Cy-NiSe and Cy-TfSe, which show weak fluorescence due to donor-excited photoinduced electron transfer, release near-infrared fluorescent dye Cy7-MeAm on cleavage of the Se-N bond by thiols. Thus, they can be used for real-time imaging of thiols such as glutathione in living cells and tissue (see figure). Copyright
