1402818-90-9Relevant articles and documents
Characterization of a highly Cu2+-selective fluorescent probe derived from rhodamine B
Yu, Chunwei,Wang, Ting,Xu, Ke,Zhao, Jing,Li, Maohua,Weng, Shixing,Zhang, Jun
, p. 38 - 44 (2013)
A rhodamine B derivative was synthesized and characterized as a highly selective and sensitive probe for Cu2+ in ethanol-water solution (2:3, v:v, pH7.4, 50 mM HEPES). A prominent fluorescence enhancement at 575 nm was observed in the presence of Cu2+, accompanied by the change in the absorption spectrum. Under the optimal conditions, a good linear range of 0.5-1.5 μM with a detection limit of 1.6 × 10-7 M were obtained. Furthermore, confocal laser scanning microscopy experiments have proven that this probe is cell-permeable and can respond to changes in intracellular Cu2+ in living cells.