140430-56-4Relevant articles and documents
Substrate-dependent activation of thermolysin by salt
Oneda, Hiroshi,Muta, Yuko,Inouye, Kuniyo
, p. 1811 - 1813 (2004)
Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu- [N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH 2 [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at X M NaCl was expressed as 4.7 x when [NaCl]0 x when [NaCl]0 > 0.5 M respectively.
Effects of salts on the interaction of 8-anilinonaphthalene 1-sulphonate and thermolysin
Samukange, Vimbai,Kamo, Masayuki,Yasukawa, Kiyoshi,Inouye, Kuniyo
, p. 1522 - 1528 (2014)
Neutral salts activate and stabilize thermolysin. In this study, to explore the mechanism, we analyzed the interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and thermolysin by ANS fluorescence. At pH 7.5, the fluorescence of ANS increased and blue-shifted with increasing concentrations (0-2.0 μM) of thermolysin, indicating that the anilinonaphthalene group of ANS binds with thermolysin through hydrophobic interaction. ANS did not alter thermolysin activity. The dissociation constants (Kd) of the complex between ANS and thermolysin was 33 ± 2 μM at 0 M NaCl at pH 7.5, decreased with increasing NaCl concentrations, and reached 9 ± 3 μM at 4M NaCl. The Kd values were not varied (31-34 μM) in a pH range of 5.5-8.5. This suggests that at high NaCl concentrations, Na+ and/or Cl- ions bind with thermolysin and affect the binding of ANS with thermolysin. Our results also suggest that the activation and stabilization of thermolysin by NaCl are partially brought about by the binding of Na+ and/or Cl- ions with thermolysin.
