1410880-22-6 Usage
Uses
JNK-IN-8 has been used as an inhibitor to address the importance of JNK signaling in withaferin A (WFA)-induced apoptosis of myelodysplastic syndromes (MDS)-L cells.
Biological Activity
jnk-in-8 is a specific jnk1/2/3 inhibitor with ic50 value of 4.67, 18.7, 0.98 nm respectively [1].c-jun n-terminal kinase (jnk) 1, 2 and 3 belong to the mitogen-activated protein kinase (mapk) family, which are able to phosphorylate c-jun on the ser63 and ser73 residue.they are responsive for stress stimuli, including cytokines and heat shock, and get involved in t cell differentiation and cell apoptosis process. jnk 1 and 2 are ubiquitous in all cell types but jnk 3 is only found in cells of brain, heart and testes tissues.jnk-in-8 is a jnk1/2/3 inhibitor with high specificity. when jnk-in-8 was profiled with a panel of 400 kinases, it exhibited specific binding to jnk 1/2/3 but not to other kinases. crystallization study also found that jnk-in-8 forms covalent bonds with conserved cysteine residue of jnk 1/2/3, resulting in a conformational change of the activation loop that blocks the substrate binding, thereby inhibiting the activity of jnk 1/2/3 [1].in hela cells and a375 cells, pretreatment of cells with jnk-in-8 resulted in the inhibition of c-jun which is a direct phosphorylation substrate of jnk 1/2/3, confirming the inhibitory action of jnk-in-8 on jnk 1/2/3. in hek293-ilr1 cells following stimulation by anisomycin, the jnk-in-8 was observed to inhibit c-jun but not msk1 and p38, and the inhibition was not reversible by removing jnk-in-8 from culture medium. additionally, jnk-in-8 only exhibited on-pathway inhibition of jnk signaling pathway, which can be monitored by the phosphorylation of c-jun [1].
Biochem/physiol Actions
JNK-IN-8 is a potent, selective and irreversible inhibitor of JNK1/2/3 that inhibits phosphorylation of c-Jun. JNK-IN-8 forms covalent bonds with a conserved cysteine residue.
references
[1]. zhang t et al., discovery of potent and selective covalent inhibitors of jnk. chemical biology. 2012, 19(1):140-154.
Check Digit Verification of cas no
The CAS Registry Mumber 1410880-22-6 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,4,1,0,8,8 and 0 respectively; the second part has 2 digits, 2 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 1410880-22:
(9*1)+(8*4)+(7*1)+(6*0)+(5*8)+(4*8)+(3*0)+(2*2)+(1*2)=126
126 % 10 = 6
So 1410880-22-6 is a valid CAS Registry Number.
1410880-22-6Relevant articles and documents
INHIBITORS OF C-JUN-N-TERMINAL KINASE (JNK)
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Paragraph 00334; 00344, (2013/06/05)
The present invention provides novel compounds according to Formula (I): where Ring A, Ring B, X, L1, L2, RA, RC, RD, RE, m, n, and p are as defined herein. Compounds of the present invention are contemplated useful for the prevention and treatment of a variety of human diseases associated with kinase activity, for example, proliferative diseases, neurodegenerative diseases, metabolic disorders, inflammatory diseases, and cardiovascular diseases.