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1414-45-5 Usage


Nisin, a type-A lantibiotic, is a ribosomally synthesized peptide with broad-spectrum antibacterial activity. It is produced by the Lactococcus lactis and contains 34 amino acid residues, including lanthionine, methyllanthionine, didehydroalanine, and didehydroaminobutyric acid, along with five thioether bridges. Nisin serves as a natural, antibacterial, and toxicologically safe food preservative, protecting against many bacteria that are food-spoilage pathogens.


Used in Food Industry:
Nisin is used as a natural preservative for inhibiting the growth of spoilage and pathogenic bacteria, particularly Gram-positive bacteria. It is effective in various food products, such as milk, meats, fast food, beverages, and health care products, to improve food quality, reduce sterilization time, lower temperature during food sterilization, lessen damage to nutrition, and extend shelf life.
Used in Cheese Production:
Nisin is used as an antimicrobial agent to inhibit the outgrowth of Clostridium botulinum spores and toxin formation in pasteurized cheese spreads, pasteurized process cheese spreads, and cheese spreads with fruits, vegetables, or meats.
Used in Pharmaceutical Industry:
Nisin has been studied as a possible treatment for infections of Clostridium difficile, particularly when combined with miconazole.
Used in Antimicrobial Applications:
As an antibiotic with bactericidal action, Nisin is particularly effective against Clostridium botulinum and is allowed in processed cheese food. It functions by binding to cell wall precursor lipid components of bacteria and disrupting cell wall production, leading to bacterial inhibition or death.


https://en.wikipedia.org/wiki/Nisin http://www.innovateus.net/food/natural-preservative-nisin http://www.sigmaaldrich.com/catalog/product/sigma/n5764?lang=en?ion=US&gclid=CPu1xf6aq9MCFcSOfgodvmcHZA

Biological Activity

nisin is an antibacterial peptide.nisin is produced by the lactic acid bacterium l. lactis using uncommon amino acids, including lanthionine, and is a member of the class of antibiotics referred to as lantibiotics.

Biochem/physiol Actions

Nisin (Nisaplin) is produced by Lactococcus lactis. It is an effective lantibiotic peptide bactericidal agent. Nisin is a broad spectrum antimicrobial bacteriocin which affects Gram-positive bacteria, however, it has little or no effect on Gram-negative bacteria, yeasts or fungi.It was demonstrated that nisin has capability to inhibit the growth of almost all tested Gram-positive beer-spoilage bacteria. Of 122 Gram-positive bacteria strains (predominantly Lactobacilli and Pediococci), 93% were sensitive to 100 units of nisin. In contrast, out of the 32 Gram-negative strains tested, only the genus Flavobacter proved to be affected by nisin.Nisin exerts its antimicrobial activity by binding to lipid II which is a membrane-bound advanced intermediate involved in the biosynthesis of bacterial cell wall. By doing so, nisin sequesters lipid II from its functional location. In addition, the nisin-lipid II complex leads to formation of pores in the membrane causing cell death.

in vitro

previous study showed that the sperm motility could be completely inhibited with nisin the minimum effective concentration of nisin required to immobilize sperm in vitro within 20 s was found to be 50 μg in rat, 200 μg in rabbit and 300-400 μg in monkey and human. such inhibitory effect on sperm motility was found to be dose- and time-dependent [1].

in vivo

intravaginal administration of nisin before mating during proestrus-estrous transition phase caused complete arrest of sperm motility and blockage of conception. subacute toxicity studies in rats showed that, repetitive intravaginal application of nisin at the dose of 200 microg for 14 days caused no abnormalities either in the length of estrous cycle or in the morphology of vaginal epithelial cells. moreover, no histopathological abnormalities in vaginal tissue or any change in blood and serum biochemical profiles were seen. in addition, no adverse effects were found on subsequent reproductive performance, development of pups and neonate survival [1].

Purification Methods

This polypeptide from S. lactis is purified by crystallisation from 80% (v/v) EtOH and by countercurrent distribution. The synthetic polypeptide antibiotic can also be purified by preparative HPLC and assayed by HPLC on a Nucleosil 3007C18 (6 x 250mm) column using a MeCN—0.01M HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5mL/minute to give a retention time of 8.1 minutes; or MeCN—0.3M guanidine-HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5ml/minute to give a retention time of 10.9minutes. FAB-MS gave the pseudomolecular ion m/z at 3352.7 (M + H)+. It is soluble in dilute acid and is stable even on boiling. [Berridge et al. Biochem J 52 529 1952, synthesis by Fukase et al. Tetrahedron Lett 29 795 1988.]


[1] aranha c, gupta s, reddy kv. contraceptive efficacy of antimicrobial peptide nisin: in vitro and in vivo studies. contraception. 2004 apr;69(4):333-8.

Check Digit Verification of cas no

The CAS Registry Mumber 1414-45-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,4,1 and 4 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 1414-45:
55 % 10 = 5
So 1414-45-5 is a valid CAS Registry Number.



According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 14, 2017

Revision Date: Aug 14, 2017


1.1 GHS Product identifier

Product name nisin

1.2 Other means of identification

Product number -
Other names -

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:1414-45-5 SDS

1414-45-5Upstream product

1414-45-5Downstream Products

1414-45-5Related news

Structural change in GadD2 of Listeria monocytogenes field isolates supports Nisin (cas 1414-45-5) resistance09/30/2019

The lantibiotic nisin is used as a food additive to effectively inactivate a broad spectrum of Gram-positive bacteria such as Listeria monocytogenes. In total, 282 L. monocytogenes field isolates from German ready-to-eat food products, food-processing environments and patient samples and 39 List...detailed

Protective effect of β-cyclodextrin on stability of Nisin (cas 1414-45-5) and corresponding interactions involved09/29/2019

Nisin has been extensively employed in food industry as an antimicrobial peptide, but the proteolytic degradation and interference by food components resulted in the decrease of its antimicrobial activity. This study intends to explore the protective effect of β-cyclodextrin (β-CD) on nisin an...detailed

Controlled release of Nisin (cas 1414-45-5) from polyvinyl alcohol - Alyssum homolocarpum seed gum composite films: Nisin (cas 1414-45-5) kinetics10/01/2019

Antimicrobial biodegradable films based on PVA-AHSG incorporated with nisin were prepared. These films were characterized for their ability to control the overall release rate of nisin into foods, which would be needed for active food packaging. The objectives of this study were to assay the bio...detailed

Spray-drying microencapsulation of Nisin (cas 1414-45-5) by complexation with exopolysaccharides produced by probiotic Bacillus tequilensis-GM and Leuconostoc citreum-BMS09/26/2019

This work aims to encapsulate nisin by complexation with exopolysaccharides (EPS), produced by Bacillus tequilensis-GM and Leuconostoc citreum-BMS, namely EPS-GM and EPS-BMS, respectively, using spray-drying technique, and to evaluate the effect of this encapsulation on the structure of nisin. R...detailed

Bypassing lantibiotic resistance by an effective Nisin (cas 1414-45-5) derivative09/25/2019

The need for new antibiotic compounds is rising and antimicrobial peptides are excellent candidates to fulfill this object. The bacteriocin subgroup lantibiotics, for example, are active in the nanomolar range and target the membranes of mainly Gram-positive bacteria. They bind to lipid II, inhi...detailed

Influence of Nisin (cas 1414-45-5) hinge-region variants on lantibiotic immunity and resistance proteins09/24/2019

The rising existence of antimicrobial resistance, confirms the urgent need for new antimicrobial compounds. Lantibiotics are active in a low nanomolar range and represent good compound candidates. The lantibiotic nisin is well studied, thus it is a perfect origin for exploring novel lantibiotics...detailed

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